PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G17750.1
Common NameATHSF1, ATHSFA1A, dl4910c, FCAALL.107, HSF1, HSF13, HSFA1A
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 495aa    MW: 55744 Da    PI: 4.8908
Description heat shock factor 1
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G17750.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind117.67.6e-37531442102
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k+y+++ed++++ ++sws ++nsf+v+d+ ef++++LpkyFkh+nf+SFvRQLn+YgF+kv+ ++         weF+++ F +g+k+ll+ki 
   AT4G17750.1  53 FLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDR---------WEFANEGFLRGQKHLLKKIS 140
                   9********************999*****************************************999.........******************** PP

                   XXXX CS
  HSF_DNA-bind  99 rkks 102
                   r+ks
   AT4G17750.1 141 RRKS 144
                   **97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM004151.1E-6349142IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene3DG3DSA:1.10.10.103.0E-4050142IPR011991Winged helix-turn-helix DNA-binding domain
SuperFamilySSF467851.12E-3550142IPR011991Winged helix-turn-helix DNA-binding domain
PRINTSPR000561.2E-205376IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004476.0E-3353142IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.2E-2091103IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS00434092116IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.2E-20104116IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009408Biological Processresponse to heat
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000084anatomyplant sperm cell
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001017developmental stageM germinated pollen stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 495 aa     Download sequence    Send to blast
MFVNFKYFSF FIRTKMDGVT GGGTNIGEAV TAPPPRNPHP ATLLNANSLP PPFLSKTYDM  60
VEDPATDAIV SWSPTNNSFI VWDPPEFSRD LLPKYFKHNN FSSFVRQLNT YGFRKVDPDR  120
WEFANEGFLR GQKHLLKKIS RRKSVQGHGS SSSNPQSQQL SQGQGSMAAL SSCVEVGKFG  180
LEEEVEQLKR DKNVLMQELV KLRQQQQTTD NKLQVLVKHL QVMEQRQQQI MSFLAKAVQN  240
PTFLSQFIQK QTDSNMHVTE ANKKRRLRED STAATESNSH SHSLEASDGQ IVKYQPLRND  300
SMMWNMMKTD DKYPFLDGFS SPNQVSGVTL QEVLPITSGQ SQAYASVPSG QPLSYLPSTS  360
TSLPDTIMPE TSQIPQLTRE SINDFPTENF MDTEKNVPEA FISPSPFLDG GSVPIQLEGI  420
PEDPEIDELM SNFEFLEEYM PESPVFGDAT TLENNNNNNN NNNNNNNNNN NNNTNGRHMD  480
KLIEELGLLT SETEH
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ldu_A2e-285114218120Heat shock factor protein 1
5d5u_B2e-285114227129Heat shock factor protein 1
5d5v_B2e-285114227129Heat shock factor protein 1
5d5v_D2e-285114227129Heat shock factor protein 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.329710.0flower
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306841740.0
Genevisible245379_at0.0
Expression AtlasAT4G17750-
AtGenExpressAT4G17750-
ATTED-IIAT4G17750-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Constitutively expressed. {ECO:0000269|PubMed:7948881}.
Functional Description ? help Back to Top
Source Description
TAIRnative protein is a trimer, interacts with HSP70, also with TBP, DNA interaction is modulated by phosphorylation and is heat-shock inducible
UniProtTranscriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
Function -- GeneRIF ? help Back to Top
  1. Results revealed that overexpressing HsfA1a had positive effects on tolerance to diverse stressors by promoting inducible Hsp expression following stress-induced HsfA1a activation.
    [PMID: 24634180]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G17750.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By heat stress. {ECO:0000269|PubMed:7948881}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G56600(A), AT2G22240(A), AT2G47180(A), AT5G12030(A)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G17750, AT5G16820
IntActSearch P41151
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G17750
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2293470.0AK229347.1 Arabidopsis thaliana mRNA for heat shock transcription factor HSF1, partial cds, clone: RAFL16-59-L13.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_193510.10.0heat stress transcription factor A-1a
SwissprotP411510.0HFA1A_ARATH; Heat stress transcription factor A-1a
TrEMBLD7M9G00.0D7M9G0_ARALL; Heat shock factor
STRINGAT4G17750.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM151011619
Representative plantOGRP9617233
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Zhang L,Eggers-Schumacher G,Sch
    Analysis of heat-shock transcription factor-DNA binding in Arabidopsis suspension cultures by UV laser crosslinking.
    Plant J., 2001. 28(2): p. 217-23
    [PMID:11722765]
  4. Kim BH,Schöffl F
    Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins.
    J. Exp. Bot., 2002. 53(367): p. 371-5
    [PMID:11807141]
  5. Zhang L,Lohmann C,Pr
    Heat stress-dependent DNA binding of Arabidopsis heat shock transcription factor HSF1 to heat shock gene promoters in Arabidopsis suspension culture cells in vivo.
    Biol. Chem., 2003. 384(6): p. 959-63
    [PMID:12887064]
  6. Wunderlich M,Werr W,Sch
    Generation of dominant-negative effects on the heat shock response in Arabidopsis thaliana by transgenic expression of a chimaeric HSF1 protein fusion construct.
    Plant J., 2003. 35(4): p. 442-51
    [PMID:12904207]
  7. Lohmann C,Eggers-Schumacher G,Wunderlich M,Sch
    Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis.
    Mol. Genet. Genomics, 2004. 271(1): p. 11-21
    [PMID:14655047]
  8. Czarnecka-Verner E,Pan S,Salem T,Gurley WB
    Plant class B HSFs inhibit transcription and exhibit affinity for TFIIB and TBP.
    Plant Mol. Biol., 2004. 56(1): p. 57-75
    [PMID:15604728]
  9. Busch W,Wunderlich M,Sch
    Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana.
    Plant J., 2005. 41(1): p. 1-14
    [PMID:15610345]
  10. Baniwal SK, et al.
    Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors.
    J. Biosci., 2004. 29(4): p. 471-87
    [PMID:15625403]
  11. Fu S,Rogowsky P,Nover L,Scanlon MJ
    The maize heat shock factor-binding protein paralogs EMP2 and HSBP2 interact non-redundantly with specific heat shock factors.
    Planta, 2006. 224(1): p. 42-52
    [PMID:16331466]
  12. Nishizawa A, et al.
    Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress.
    Plant J., 2006. 48(4): p. 535-47
    [PMID:17059409]
  13. Liu HT, et al.
    Calmodulin-binding protein phosphatase PP7 is involved in thermotolerance in Arabidopsis.
    Plant Cell Environ., 2007. 30(2): p. 156-64
    [PMID:17238907]
  14. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  15. Liu HT, et al.
    The calmodulin-binding protein kinase 3 is part of heat-shock signal transduction in Arabidopsis thaliana.
    Plant J., 2008. 55(5): p. 760-73
    [PMID:18466301]
  16. Nishizawa A,Yabuta Y,Shigeoka S
    Galactinol and raffinose constitute a novel function to protect plants from oxidative damage.
    Plant Physiol., 2008. 147(3): p. 1251-63
    [PMID:18502973]
  17. Guo L, et al.
    Isolation of heat shock factor HsfA1a-binding sites in vivo revealed variations of heat shock elements in Arabidopsis thaliana.
    Plant Cell Physiol., 2008. 49(9): p. 1306-15
    [PMID:18641404]
  18. Li M, et al.
    Detection of in vivo interactions between Arabidopsis class A-HSFs, using a novel BiFC fragment, and identification of novel class B-HSF interacting proteins.
    Eur. J. Cell Biol., 2010 Feb-Mar. 89(2-3): p. 126-32
    [PMID:19945192]
  19. Banti V,Mafessoni F,Loreti E,Alpi A,Perata P
    The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis.
    Plant Physiol., 2010. 152(3): p. 1471-83
    [PMID:20089772]
  20. Xu X, et al.
    Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress.
    Biotechnol. Lett., 2010. 32(7): p. 979-87
    [PMID:20229063]
  21. Hsu SF,Lai HC,Jinn TL
    Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis.
    Plant Physiol., 2010. 153(2): p. 773-84
    [PMID:20388662]
  22. Li M,Berendzen KW,Sch
    Promoter specificity and interactions between early and late Arabidopsis heat shock factors.
    Plant Mol. Biol., 2010. 73(4-5): p. 559-67
    [PMID:20458611]
  23. Shin R,Jez JM,Basra A,Zhang B,Schachtman DP
    14-3-3 proteins fine-tune plant nutrient metabolism.
    FEBS Lett., 2011. 585(1): p. 143-7
    [PMID:21094157]
  24. Yoshida T, et al.
    Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression.
    Mol. Genet. Genomics, 2011. 286(5-6): p. 321-32
    [PMID:21931939]
  25. Liu HC,Charng YY
    Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response.
    Plant Signal Behav, 2012. 7(5): p. 547-50
    [PMID:22516818]
  26. Liu Y, et al.
    Arabidopsis heat shock factor HsfA1a directly senses heat stress, pH changes, and hydrogen peroxide via the engagement of redox state.
    Plant Physiol. Biochem., 2013. 64: p. 92-8
    [PMID:23399534]
  27. Qian J, et al.
    Overexpression of Arabidopsis HsfA1a enhances diverse stress tolerance by promoting stress-induced Hsp expression.
    Genet. Mol. Res., 2014. 13(1): p. 1233-43
    [PMID:24634180]
  28. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  29. Lee JH,H
    Derepression of the activity of genetically engineered heat shock factor causes constitutive synthesis of heat shock proteins and increased thermotolerance in transgenic Arabidopsis.
    Plant J., 1995. 8(4): p. 603-12
    [PMID:7496404]
  30. H
    Arabidopsis heat shock factor is constitutively active in Drosophila and human cells.
    Mol. Gen. Genet., 1995. 248(2): p. 136-41
    [PMID:7651336]
  31. H
    Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein.
    Plant Mol. Biol., 1994. 26(1): p. 353-62
    [PMID:7948881]
  32. Reindl A,Schöffl F,Schell J,Koncz C,Bakó L
    Phosphorylation by a cyclin-dependent kinase modulates DNA binding of the Arabidopsis heat-shock transcription factor HSF1 in vitro.
    Plant Physiol., 1997. 115(1): p. 93-100
    [PMID:9306693]
  33. Reindl A,Schöffl F
    Interaction between the Arabidopsis thaliana heat shock transcription factor HSF1 and the TATA binding protein TBP.
    FEBS Lett., 1998. 436(3): p. 318-22
    [PMID:9801140]